Rostafuroxin ameliorates cardiac glycoside-induced cardiomyocyte electrolyte imbalances and arrhythmia in ovo
Year: 2026
Type: article
Abstract: This study evaluates rostafuroxin, a selective ouabain inhibitor, for its potential to antagonize electrophysiological derangements in ouabain overdosing. Methodologically, the study uses the iCE model, previously introduced as a suitable 3 R-compliant cardiovascular research platform. We developed and validated a comprehensive electrophysiological workflow in iCEs to perform our investigations. Ouabain increased heart rate variability, induced arrhythmia and electrolyte imbalances in iCEs, whereas rostafuroxin largely protected them from these effects.
Authors: Judith Rees, Annika Winkler, Judith Huettemeister, Laura Stengel, Paul Spangler, Girish Ramesh, Justus Kamp, Teresa Charlotte Funk-Hilsdorf, Laura Michalick, Anna-Lena Herm, Peter Maximilian Deissler, Alexandr Melnikov, Michael Höpfner, Felix Hohendanner, Claudia Crocini, Callum Zgierski-Johnston, Steffen Pabel, Bianca Nitzsche, Jana Grune, Niklas Hegemann
Institutions: Humboldt-Universität zu Berlin, Deutsches Herzzentrum der Charité, German Centre for Cardiovascular Research, Freie Universität Berlin, Charité - Universitätsmedizin Berlin, Justus-Liebig-Universität Gießen, University of Freiburg, Maastricht University Medical Centre, Maastricht University, Massachusetts General Hospital, German Center for Lung Research
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7128014005