Natural extracts and bioactive compounds against L3 larvae of Anisakis spp: in vitro and ex vivo evaluation and potential application in marinated products
Year: 2026
Type: article
Abstract: This work investigated the efficacy of rosemary essential oil (REO) and Boswellia serrata extracts (BSE) against Anisakis larvae for possible industrial applications. In-vitro assays exposed Anisakis type I larvae to various media containing REO (1-5%) or BSE (1-5%) with spermidine and ferulic acid at different temperatures and times. Ex-vivo trials in experimentally infested anchovy fillets were carried out only for REO. REO demonstrated significant anisakicidal activity, achieving the fastest efficacy in oil (15h at 4 °C with 5% REO). Lower concentrations (1%) showed limited effectiveness. BSE achieved a maximum 92% larval devitalisation at 5%. Ex-vivo experiments revealed initial devitalisation after 72h, reaching 100% by 168h in anchovy fillets with 5% REO-oil at 20 °C. REO exhibited substantial anisakicidal effects in marinated anchovies under refrigeration and lipid conditions. These findings highlight REO as a promising natural additive for enhancing marinated fish product safety, offering an alternative or complement to freezing for Anisakis risk management.
Source: Figshare
Language: English
Subfield: Food Science
Domain: Life Sciences
SDGs: Zero hunger, Life below water
Open Access status: green
OpenAlex ID: W7128056094