Galanin Inhibits Histaminergic Neurons via Galanin Receptor 1
Year: 2026
Type: article
Abstract: Galanin-expressing neurons in the ventrolateral preoptic area (VLPO galanin ) are active during sleep and play an important role in regulating non-rapid eye movement (NREM) sleep. It is generally believed that VLPO galanin neurons promote sleep via inhibitory actions in arousal-promoting regions of the brain. Histaminergic neurons are a population of wake-active neurons that receive strong projections from the sleep-active VLPO galanin neurons. However, the ability of galanin to influence the activity of histaminergic neurons has received limited attention. Here, using whole-cell patch-clamp electrophysiological recordings from genetically identified histaminergic neurons in male mice, we explore the mechanisms by which galanin influences histaminergic neuron electrical excitability. Our results reveal that galanin is a powerful inhibitor of histaminergic neuron activity and demonstrate that the inhibitory effects of galanin are mediated by galanin receptor 1 (GALR1) and the subsequent opening of G-protein-coupled inwardly rectifying (GIRK) and large conductance calcium-activated potassium (BK) channels. Furthermore, we identify that histaminergic neurons highly express Galr1 mRNA and show that the GALR1-mediated hyperpolarization of histaminergic neurons is largely independent of action potential-dependent synaptic transmission or fast excitatory or inhibitory neurotransmitters. Together, these results suggest that direct postsynaptic activation of GALR1 expressed on histaminergic neurons mediates the inhibitory effects of galanin on these neurons. This data also supports the notion that the sleep-promoting effects of VLPO galanin neuron activation may occur via the ability of galanin to inhibit the arousal-promoting histaminergic neurons.
Source: eNeuro
Language: English
Subfield: Cognitive Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,925
OpenAlex ID: W7128160713