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Cover Feature: Cas12Fold Accurately Predicts Cas12 Nuclease Structures to Enable Structure‐based Genome‐editing Engineering (Small Struct. 2/2026)

Year: 2026

Type: article

Abstract: Structure-Based Genome-Editing Engineering In their Research Article (10.1002/sstr.202500674), Zhijia Yang, Hainan Zhao, and co-workers present Cas12Fold, a deep-learning framework tailored to Cas12 nucleases, that uses evolutionary signals and iterative structure-based alignment to resolve conformational complexity. This method outperforms general models in predicting key domains and states, accurately modeling enzymes such as Casλ. Models guide mutagenesis, optimizing Cas12j.4 efficiency and enabling CRISPR–Cas12 engineering.

Source: Small Structures

DOI: 10.1002/sstr.70313

Authors: Feng Xu, Zilong Zhao, Chang Liu, Meixia Yu, Ke Li, Yilin Jing, Peiyang Li, Beibei Xin, Jian Chen, Lizhu E, Jinsheng Lai, Zhijia Yang, Hainan Zhao

Institution: China Agricultural University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $4,110

OpenAlex ID: W7128434715

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