Work

Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloids

Year: 2026

Type: article

Abstract: Organoids derived from normal human tissue have proven to be effective preclinical models for studying tumor progression through the introduction of driver mutations. In this protocol, we outline techniques for disease modeling using organoids derived from healthy kidney tissue, also known as tubuloids. We provide step-by-step instructions for the establishment and passaging of tubuloids, lentiviral transduction, processing for histological analysis, and preparation for CUT&RUN and single-cell transcriptomic profiling. For complete details on the use and execution of this protocol, please refer to Ganpat et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104362

Authors: Giulia Perticari, Maroussia M.P. Ganpat, Jarno Drost

Institutions: Oncode Institute, Princess Máxima Center, Utrecht University

Language: English

Topic: Renal and related cancers

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550