Work

Protocol for intranuclear Nrf2 detection in activated mouse, human, and Jurkat T cells by spectral flow cytometry

Year: 2026

Type: article

Abstract: Nuclear factor erythroid 2-related factor 2 (Nrf2) coordinates cellular antioxidation responses. Its expression increases in T cells upon activation. Here, we present a protocol for quantitative detection of intranuclear Nrf2 in activated mouse, human, and Jurkat T cells using spectral flow cytometry. We describe procedures for primary T cell isolation, in vitro activation, intranuclear staining, and flow cytometric acquisition and analysis, including gating strategies for CD4 + and CD8 + T cells. For complete details on the use and execution of this protocol, please refer to Tripathi et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104360

Authors: Aprajita Tripathi, Debolina Dasgupta, Mehak Ahuja, Gabrielle Fisette, Nadine Santana-Magal, Kalyani Pyaram

Institution: University of Kansas Medical Center

Language: English

Topic: Genomics, phytochemicals, and oxidative stress

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550