Work

Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert

Year: 2026

Type: article

Abstract: This study is the first to utilize nano-scale Qodume Shirazi seed mucilage (QSSM) and Camelina sativa seed mucilage (CSSM), individually and in combination, as natural wall materials for microencapsulation of Lacticaseibacillus rhamnosus (LR). SEM analysis indicated smooth bead surfaces and successful entrapment of LR. The 50:50 QSSM: CSSM formulation exhibited 97.92% encapsulation efficiency, and microencapsulated LR (MLR) remained viable (>6 log CFU/g) after 15 min at 72 °C. Moreover, MLR maintained viability above 7.85 log CFU/mL for 21 days at 4 °C in goat milk dessert (GMD). Under simulated gastrointestinal conditions MLR demonstrated a 57.53% survival rate compared with free LR, which was 47.17% at the end of storage. The addition of MLR increased the hardness (258.0 ± 16.0 g) and gumminess (102.56 ± 8.45 g) of GMD. Overall, the results demonstrate that QSSM and CSSM mucilages can serve as effective natural encapsulating agents, enhancing the thermal and storage stability.

Source: Biotechnology Reports

DOI: 10.1016/j.btre.2026.e00950

Authors: Seyed Saeed Sekhavatizadeh, Neda Maftoonazad, Hadis Motashafi, Mehdi Nikkhah, Maryam Asnaashari

Institution: Agricultural Research & Education Organization

Language: English

Topic: Microencapsulation and Drying Processes

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,480