Work

Myosin Class I Genes Follow a Cell Type-Specific Transcription Pattern in Human Haematopoietic Cell Lines

Year: 2026

Type: article

Abstract: Class I myosins form an abundant group of single-headed motor proteins that interact with actin and membrane phospholipids and are employed in a variety of cell processes like cell shape remodeling, motility and invasion, exocytosis, and endocytosis. The large number of class members and their uneven distribution in human cells and tissues complicate their study. Furthermore, this hinders the assessment of their biomarker potential and mechanistic role in oncogenesis and other diseases. Despite the emerging data on their mutations and altered abundance in a number of tumors and other diseases, most studies examine class I myosins individually or in groups of several proteins. Thus, the question of their redundancy and possible functional substitution is ignored. In this work, we simultaneously assess all eight human MYO1 genes on cell lines of different origins by RNA-seq re-analysis. We also report a qPCR system for human myosin I screening and test it on a set of 35 continuous cell lines widely used in biomedicine and fundamental research. These data clarify the restricted expression patterns of MYO1 genes in various human cell types and allow to assess the pattern specific of haematopoietic cell lines.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms27041777

Authors: Alexandra E. Golysheva, Anna V. Tvorogova, Artyom A. Tyukaev, Aleena A. Saidova, Daria M. Potashnikova

Institutions: Lomonosov Moscow State University, Shenzhen MSU-BIT University

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,499