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ERGA-BGE reference genome of Gambusia holbrooki, a globally invasive freshwater fish

Year: 2026
Type: article
Abstract: The Gambusia holbrooki (eastern mosquitofish) reference genome will offer a crucial resource for understanding the evolution and adaptation of invasive freshwater fish species. The genome of G. holbrooki was assembled into two haplotypes through a phased assembly approach; however, only the primary haplotype was designated as the reference genome for annotation and downstream analyses. The entirety of the genome sequence was assembled into 24 contiguous chromosomal pseudomolecules and 1 mitochondrial genome. This chromosome-level assembly encompasses 0.67 Gb, composed of 421 contigs and 318 scaffolds, with contig and scaffold N50 values of 15.9 Mb and 29.6 Mb, respectively.
DOI: 10.12688/openreseurope.22693.1
Authors: Bonnie Fraser, Clelia Gasparini, Francesco Santi, Astrid Böhne, Rita Monteiro, Thomas Marcussen, Rebekah A. Oomen, Torsten H. Struck, Amy Denton, Caroline Howard, Kerstin Howe, Mark Blaxter, Shane McCarthy, Jonathan M.D. Wood, Fergal Martin, Swati Sinha, Leanne Haggerty, Chiara Bortoluzzi
Institutions: University of Exeter, University of Padua, Leibniz Institute for the Analysis of Biodiversity Change, University of Oslo, University of New Brunswick, University of Agder, University of Gothenburg, Wellcome Sanger Institute, European Bioinformatics Institute, SIB Swiss Institute of Bioinformatics
Language: English
Open Access status: gold
Estimated APC paid: $841
OpenAlex ID: W7128795503
FWCI:
Cites: 29
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