Work

Development and comparative study of rapid detection methods for Salmonella based on multiple molecular and immunological techniques

Year: 2026

Type: article

Abstract: The detection of Salmonella in milk is critical for dairy safety, requiring rapid and sensitive methods. This study developed and compared three independent techniques: real-time PCR (qPCR), loop-mediated isothermal amplification (LAMP), and a gold immunochromatographic test strip (GICT), all targeting the invA gene. In artificially contaminated milk, qPCR achieved a detection limit of 10 3 CFU/mL with no cross-reactivity against non-target bacteria. LAMP amplification was completed within 30 min at 63°C, with detection limits of 4.17×10 3 CFU/mL (visual) and 4.17×10 2 CFU/mL (gel electrophoresis). The GICT strip, based on monoclonal antibody 8E7 1C8, showed a detection limit of 10 4 CFU/mL and remained stable for six months at 4°C. While qPCR and LAMP offered high sensitivity suitable for laboratory quantification, GICT provided a rapid, equipment-free option for on-site screening. Together, these methods provide a complementary toolkit for detecting Salmonella across laboratory and field settings.

Source: International Dairy Journal

DOI: 10.1016/j.idairyj.2026.106582