Quantification of Aspergillus nidulans Actin Dynamics during Early Growth and Septum Formation
Year: 2026
Type: preprint
Abstract: Filamentous fungi have complex, three-dimensional growth patterns and a non-adherent nature, which can present challenges for live-cell imaging for quantitative assessment of dynamic cellular processes. To address these challenges, a live-cell imaging system has been modified to constrain the model fungus Aspergillus nidulans to growth in a single focal plane. This enables high-resolution time-lapse imaging of actin dynamics throughout development using a Lifeact actin marker. This system was used to perform kymographic analysis to quantify actin velocity and hyphal extension rates during early hyphal development. Results show two distinct growth phases: germ tube extension (0.58 μm/min) and hyphal extension (1.52 μm/min). Actin exhibited bi-directional transport along hyphae with biased movement toward the spore body. Actin was also observed re-localizing from hyphal tips to sites of septum formation indicating active redistribution of cytoskeletal resources based on cellular demands. This technological advancement overcomes longstanding limitations in fungal live-cell imaging and provides a new platform for quantitative systems-level analysis of mycelial development, offering new insights into the spatiotemporal coordination of cytoskeletal dynamics during filamentous growth.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: Collaborative Research: SEALing the mold: systems analysis of the fungal response to cell wall damage, Collaborative Research: SEALing the mold: systems analysis of the fungal response to cell wall damage, Collaborative Research: Using multi-omic analyses and dynamic modeling to understand fungal cell-wall stress responses
OpenAlex ID: W7128813079