Modulation of Contactless High Intensity Pulsed Electromagnetic Field Induced Electroporation and Gene Delivery Efficacy Using Various Nanoparticles
Year: 2026
Type: article
Abstract: Introduction: are currently inferior to EP. It was suggested that the methodology can be improved with conductive gold nanoparticles (AuNPs), which are reported to amplify the induced electric field in close proximity to the cell membrane. Objectives: Therefore, in this work, we have studied different NPs, which varied in material/conductivity (gold and silica), size (10-50+ nm), shape (i.e., round and rods), concentration (50-200 µg/mL), and functionalization (pegylated or not), and combined them with HI-PEMF (6.7 T × 100 pulses, 1 Hz). Methods: The normal Chinese hamster ovary cell line (CHO) and the cancer human urinary bladder's transitional carcinoma cell line (T24) were used as a model. We have characterized cell membrane permeabilization using propidium iodide (PI) and the efficacy of gene delivery using pEGFP-N1. Results: Larger NPs and higher NP concentrations resulted in up to a 10% increase in membrane permeability. In contrast, semispherical and rod-shaped AuNPs did not further enhance permeabilization efficiency. Gene delivery efficiency increased from 3% in control samples to 6% in the presence of 50 nm AuNPs. Overall, CHO cells were more susceptible to HI-PEMF-induced effects than T24 cells. Conclusions: This study shows the potential to increase gene delivery efficacy by combining HI-PEMF treatment with conductive NPs. However, it was concluded that the HI-PEMF-induced effects are highly dependent on the cell line, NP type, and concentration and therefore require further investigation.
Source: Bioelectricity
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: green
Awards: Cell Biology and Molecular Genetics in Biomedicine, Electroproation-based technologies and treatments, Enhancing heat exchanger performance using advanced superhydrophobic coating; Super-COR-AI, The Infrastructure Program of University of Ljubljana »Network of Research and Infrastructural Centers UL (MRIC UL)«, Selective electroporation by distributed nanoelectrodes, Bactericidal nanoblades: a proof-of-concept approach for bimodal chemo-mechanical eradication of persistent biofilms, Selective mechanical removal of bacterial biofilms by conjugated magnetic nanoparticles, Advanced magnetic and multifunctional materials, Exploitation of the magneto-mechanical effect in the treatment of neurodegenerative diseases, Magnetic micro-structuring of the Mg alloy surfaces for improved endothelialization and retained degradability of vascular stent materials, Nauja auglių pašalinimo technologija naudojant stipraus magnetinio lauko impulsų sekas valdomai vaistų ir DNR pernašai, Noninvasive Noncontact Electroporation for Efficient Gene Therapy
OpenAlex ID: W7128923680