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Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma

Year: 2026

Type: article

Abstract: Whole-genome sequencing (WGS) has replaced Pulsed-field gel electrophoresis (PFGE)-based bacterial genotyping as the reference genotyping method. We investigated the suitability of purified genomic DNA extracted from PFGE agarose plugs stored in a laboratory collection for WGS in cases where bacterial isolates are no longer available. Our study has shown that bacterial WGS can be successfully performed on DNA extracted from PFGE agarose plugs.

Source: Repository of the University of Ljubljana (University of Ljubljana)

Authors: Strašek Smrdel, Katja, Celar Šturm, Andraž, Triglav, Tina, Pirš, Mateja

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W7128944093

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