Work

Protocol for isolation of total RNA from mouse whole cochlea using organic solvents

Year: 2026

Type: article

Abstract: Determining gene expression changes in cochlear components is important for understanding the mechanisms underlying hearing loss. Here, we present a protocol for the isolation of total RNA from mouse cochleae across a wide range of ages (2–25 weeks old) using organic solvent extraction. We describe the steps for obtaining the entire cochlea from mice and isolating high-quality total RNA suitable for further reverse transcription and PCR. For complete details on the use and execution of this protocol, please refer to Rías et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104376

Authors: Ezequiel Rías, Guillermo Spitzmaul, Leonardo Dionisio

Institutions: Consejo Nacional de Investigaciones Científicas y Técnicas, Centro Científico Tecnológico - Bahía Blanca, Instituto de Investigaciones Bioquímicas de Bahía Blanca, CONICET-UNS, Universidad Nacional del Sur

Language: English

Topic: Hearing, Cochlea, Tinnitus, Genetics

Subfield: Sensory Systems

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional del Sur, Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación, Secretaría General de Ciencia y Tecnología , Universidad Nacional del Sur