Protocol for establishing a mouse model of bilateral maxillary first molar extraction to study alveolar bone healing
Year: 2026
Type: article
Abstract: Alveolar bone healing following tooth extraction is a complex biological process. Here, we present a protocol for establishing a reproducible mouse model of bilateral maxillary first molar extraction to study alveolar bone healing. We describe steps for surgery, postoperative care, and tissue collection for downstream genomic, histological, and micro-computed tomography (CT) analyses. We provide practical strategies to minimize root fracture and soft tissue injury, ensuring procedural consistency and high-quality outcomes. • Steps for minimally invasive extraction of bilateral maxillary first molars in mice • Instructions for optimizing oral access using rubber bands and cotton balls • Guidance for intact tooth removal using syringe needles and micro-toothed forceps • Procedures for collecting alveolar bone tissue for histological and micro-CT analyses Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Alveolar bone healing following tooth extraction is a complex biological process. Here, we present a protocol for establishing a reproducible mouse model of bilateral maxillary first molar extraction to study alveolar bone healing. We describe steps for surgery, postoperative care, and tissue collection for downstream genomic, histological, and micro-computed tomography (CT) analyses. We provide practical strategies to minimize root fracture and soft tissue injury, ensuring procedural consistency and high-quality outcomes.
Source: STAR Protocols
Authors: Jiarui Jiang, Jiayu Mou, Siwei Wang, Jianguo Liu
Institution: Zunyi Medical University
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,550
Award: G4645600365
OpenAlex ID: W7129023932