Work

Molecular Design Strategies Enabling Activity-Based Sensing and Labeling as a Platform for Probing Enzyme Activity, Redox Regulation, and Transition-Metal Signaling

Year: 2026

Type: article

Abstract: Activity-based sensing and labeling (ABSL) has emerged as a powerful platform for the selective detection of transient biological analytes with high sensitivity and spatial fidelity. Here, we highlight emerging molecular design strategies that leverage ABSL for advanced investigations of critical cellular signaling processes. We summarize key biological insights resulting from ABSL of enzyme activity, reactive oxygen and nitrogen species (ROS/RNS), and labile transition-metal pools.

Source: Bioconjugate Chemistry

DOI: 10.1021/acs.bioconjchem.5c00662

Authors: Hanna I. Humpel, Alexander P. Luancing, Benjamin H. Shostak, Marco S. Messina

Institution: University of Delaware

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: University of Delaware Research Foundation, National Institutes of Health, National Institute of General Medical Sciences

Awards: G2353688610, G2390241213, G7089923881, Center for Biomedical Research Excellence in Multiscale Biomolecular Sensing (MBS)