Structural Basis for Trivalent Cross‐Linking of a Patient‐Derived IgE Antibody by the Major Peanut Allergen Ara h 2.0201
Year: 2026
Type: article
Abstract: BACKGROUND: Peanut allergy is a serious form of food allergy that can lead to anaphylactic reactions. Research has shown that the Ara h 2 allergen is immunodominant and associated with fatal systemic reactions. METHODS: We determined the crystal structure of recombinant Ara h 2.0201 in a complex with the human-derived PA12P3D08 (D08) IgE Fab fragment at 3.2 Å resolution. In addition, native mass spectrometry was used to study interactions of D08 with Ara h 2 and its peptides. RESULTS: The structure revealed that D08 Fab binds to a long loop of Ara h 2.0201, which contains three repeated DPYSPS motifs. The immunocomplex structure illustrated how three copies of D08 Fabs can bind simultaneously to Ara h 2.0201 in close proximity. Native mass spectrometry studies of D08 Fab with Ara h 2.0201 and peptides containing 2-3 motifs demonstrated cross-linking of Ara h 2.0201 in solution and the propensity of D08 Fab to self-associate. Motif peptides from Ara h 2.0201 highlighted the importance of proline hydroxylation for binding affinity. D08 Fab also bound to hydroxyproline-containing peptides from Ara h 1 and 3. CONCLUSIONS: The trivalent binding is effective in forming large allergen-IgE complexes on mast cell or basophil surfaces and contributes to the potency of Ara h 2 in triggering allergic reactions and highlighting its role in anaphylaxis. Proline hydroxylation considerably enhances D08 binding affinity and contributes to cross-reactivity among Ara h 1-3 allergens.
Source: Allergy
DOI: 10.1111/all.70258
Institution: University of Eastern Finland
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $5,300
Funders: Itä-Suomen Yliopisto, Biocenter Finland
OpenAlex ID: W7129519572