CaptureBody – an anti-CD45 x anti-IgG bispecific antibody enables accurate unmixing for spectral flow cytometry
Year: 2026
Type: preprint
Abstract: Accurate spectral unmixing is a critical step for flow cytometry data analysis and requires a single stain control for every fluorescent parameter used in an experiment. Currently, compensation particles are often used for making single stain controls when a target protein is of low abundance or a cell type is of low frequency. However, compensation particles introduce incongruencies in emission spectra compared to cells resulting in spectral unmixing or compensation errors. To enable the use of cells regardless of the abundance of target proteins or immune cell type, we generated a bispecific antibody that links a human anti-CD45 and mouse anti-IgG variable region. We refer to this new bispecific tool as CaptureBody (CB) and highlight the benefits of its final nanobody-based design. We provide all sequences and methods necessary for the in-house expression of a CaptureBody to disseminate their use for spectral flow cytometry experiments.
Institutions: Fred Hutch Cancer Center, University of Washington
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: The role of tissue-resident T cells in resolving inflammation of the human oral mucosa, Cancer Center Support Grant, Metabolite- and cytokine-mediated signals interact to control human CD8 T cell responses in tissues, Inflammation-driven T Cell Responses and their Dichotomous Effect on Host Immunity
OpenAlex ID: W7129645751