A biohybrid system to reconstruct epithelial morphomechanics in vitro
Year: 2026
Type: article
Abstract: Epithelial tissue compaction driven by smooth muscle contraction is central to epithelial morphogenesis and mechanotransduction. However, in vitro systems that recapitulate this mechanical process – especially those capable of mimicking the anisotropic and multi-axial nature of smooth muscle contraction – remain limited. Here, we present the bio hybrid pr ogrammable e pithelial tissue c ompaction s ystem (BIOPRECS), consisting of an overlying contraction-responsive epithelial tissue (ET) and underlying thermo-responsive contractile hydrogel (CH). By coupling isotropic hydrogel contraction with defined epithelial geometric anisotropy, BIOPRECS enables programmable multi-axial epithelial compaction that mirrors in vivo processes. Its in situ culture-compatible setup allows straightforward examination of both tissue-scale folding and subcellular deformation, including cytoplasmic and nuclear responses. Furthermore, by modulating tissue geometry and boundary conditions, BIOPRECS reproduces organ-specific epithelial architectures reminiscent of those observed in the lung, intestine, and stomach. This system has a significant potential to become a powerful tool for investigating how mechanical compaction regulates epithelial morphogenesis and mechanotransduction.
Source: Materials Today Bio
Authors: Jangwon Yoon, Jaeseung Youn, Dong Sung Kim
Institutions: Pohang University of Science and Technology, CHA University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,400
OpenAlex ID: W7129740502