Effects of New Cellular Treatment Factor and Human Exosome Injection on Intact Skin Regeneration in a Randomized In Vivo Study
Year: 2026
Type: article
Abstract: Background: Enhancing intact skin regeneration is a growing focus in dermatology and aesthetics. Human derived exosomes and mesotherapy formulations such as New Cellular Treatment Factor (NCTF) have shown regenerative potential, yet their combined effects on healthy skin remain unstudied. This study evaluated NCTF, two exosome products, and their combinations on markers of skin regeneration. Methods: A randomized, within-animal controlled study was conducted in 144 adult male Syrian golden hamsters. Each animal received 6 intradermal injections on the dorsum: saline control, CellExosome, ASCE+ Exosome, NCTF, NCTF + CellExosome, or NCTF + ASCE+ Exosome. Skin biopsies were obtained at baseline and on days 3, 7, and 14. Histological assessments (hematoxylin and eosin, Van Gieson, Masson trichrome) evaluated dermal structure and collagen deposition. CD34 immunohistochemistry quantified microvascular density. Results: < 0.0001). The NCTF + CellExosome combination produced the highest improvements in collagen density and microvascularity, followed by NCTF + ASCE+ Exosome, NCTF alone, ASCE+ Exosome, and CellExosome. These findings indicate synergistic biological effects when NCTF is combined with exosomes. Conclusions: NCTF and human-derived exosomes enhance dermal structural regeneration and angiogenesis in intact skin. Combination therapy, particularly NCTF + CellExosome, provides the strongest regenerative response, supporting further investigation for clinical aesthetic applications.
Authors: Noury Adel, Jack Kolenda
Institution: University of Toronto
Language: English
Topic: Wound Healing and Treatments
Subfield: Rehabilitation
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,195
OpenAlex ID: W7130400116