Grin2b 3’UTR is necessary for synaptic plasticity and spatial learning
Year: 2026
Type: article
Abstract: Spatially precise protein synthesis is critical for synaptic plasticity and cognitive function. The GRIN2B transcript encodes the GluN2B subunit of N-methyl-D-aspartate (NMDA) receptors and has a 25 kb-long 3’-untranslated region (3’UTR) of unknown function. To investigate its role, we generated a ∆3’UTR mouse line by deleting the Grin2b 3’UTR while preserving the coding sequence. Despite unchanged Grin2b mRNA levels in ∆3’UTR mice, GluN2B protein was reduced 50%. In wildtype (WT) mice, Grin2b mRNA was enriched in synaptosomes, but this enrichment was impaired in ∆3’UTR mice along with reduced GluN2B phosphorylation. Notably, ∆3’UTR mice had no long-term potentiation (LTP) and exhibited hippocampal-dependent spatial learning impairments. Together, these findings demonstrate that the 3’UTR is critical for Grin2b mRNA function, ultimately influencing synaptic composition and plasticity.
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Funders: Aarhus Universitet, Georgetown University, European Molecular Biology Laboratory, National Research Foundation, Danmarks Grundforskningsfond, Lundbeckfonden, Novo Nordisk, H. Lundbeck A/S, Institute for Translational Neuroscience, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
OpenAlex ID: W7130412679