Year: 2026
Type: article
Abstract: Introduction Infected diabetic wounds pose a significant clinical challenge owing to the complex wound microenvironment. Various multifunctional synergistic therapeutic nanomaterials have been successfully developed to treat diabetic infected wounds. Objective A Janus Au–porphyrin polymersome heterostructure loaded with metformin (Met@J-AuPPS) is constructed and presented. Methods Porphyrin polymersome vesicles loaded with metformin (Met@PPS) was synthesized by supramolecular polymerization enhanced self-assembly. Metformin-loaded Janus gold-porphyrin polymersome (Met@J-AuPPS) was synthesized by photocatalytic synthesis method. Transmission electron microscope (TEM), scanning electron microscope (SEM), UV-vis absorption spectrum and ELISA experiment were used to detect the morphology, structure, photothermal properties and drug release properties of Met@J-AuPPS. The effects of Met@J-AuPPS on the proliferation and activity of HUVECs were detected by CCK-8 test, scratch test, Transwell test and tube formation test. The antibacterial and antibiofilm abilities of Met@J-AuPPS were detected by blood plate test, crystal violet staining, SEM of biofilm and bacterial live/dead staining. Imaging and histological staining were used to detect the efficacy of Met@J-AuPPS in treating diabetic wound infections in vivo . Results Excellent photothermal antibacterial performance against gram-positive methicillin-resistant Staphylococcus aureus (MRSA), gram-negative extended-spectrum β-lactamases Escherichia coli (ESBL E. coli ), and MRSA/ESBL E. coli biofilm is demonstrated by a strong non-centrosymmetric near-field enhancement (NFE) effect between Met@PPS and Au nanoparticles. Furthermore, the release of metformin under near-infrared (NIR) light promotes angiogenesis and tissue repair. The results therefore show that Met@J-AuPPS exhibits excellent antibacterial/biofilm and pro-angiogenic performance, with significantly enhanced therapeutic effects in infected wounds of diabetic rat models. Conclusion This study presents an innovative therapeutic strategy for diabetic wound infections and demonstrates that Met@J-AuPPS has potential as a multifunctional and upgradeable nanoplatform for further biomedical applications.
Language: English
Topic: Wound Healing and Treatments
Subfield: Rehabilitation
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
Awards: G2460586620, G8508572310
OpenAlex ID: W7130502015