Work

Spatial transcriptomics maps early B-cell and T-cell activation in hidradenitis suppurativa

Year: 2026

Type: article

Abstract: Hidradenitis suppurativa is a chronic, debilitating inflammatory skin disease that progresses from superficial nodules in early stages to dermal tunnels, tertiary lymphoid structures, and fibrosis in advanced lesions. Interplays between dermal tunnel keratinocytes, tertiary lymphoid structures, and fibroblasts promote pathogenic T-cell and B-cell inflammation in late-stage disease. However, whether these immune activations sequentially precede or follow late-stage structure formation is unclear. To delineate the temporal sequence of immune activation, we integrated spatial and single-cell transcriptomic analyses of early- and late-stage hidradenitis suppurativa and compared them with those of acne conglobata and psoriasis. Spatial transcriptomics of 156 regions of interest revealed robust dermal immune activation in early-stage hidradenitis suppurativa, including B-cell enrichment, type 17 T-cell pathway activation, and neutrophilic infiltration, occurring prior to tunnel or tertiary lymphoid structure formation. Single-cell RNA sequencing confirmed early plasma cell expansion and identified multifunctional B cells expressing proinflammatory cytokines, antigen-presentation machinery, and Igs. Notably, T cells rather than fibroblasts were the predominant producers of the B-cell chemoattractant CXCL13 in early lesions. Together, these findings demonstrate that hidradenitis suppurativa exhibits early and sustained immune activation that precedes dermal remodeling, underscoring the aggressive nature from its onset and suggesting that early therapeutic targeting of the type 17 T-cell axis and B-cell-mediated inflammation may help prevent disease progression.

Source: Journal of Investigative Dermatology

DOI: 10.1016/j.jid.2026.01.041

Authors: Jongeun Lee, Seoyoon Ham, Jongmi Lee, James G. Krueger, Young In Lee, Jaehwan Kim