Multifunctional Alginate-Gelatin Scaffolds Containing Cerium-Doped Bioactive Glass for Potential Bone Regeneration: Physicochemical, Antioxidant, and Antibacterial Evaluations.
Year: 2026
Type: article
Abstract: Objective: RScaffolds incorporating bioactive glass (BG) have shown significant potential in promoting bone tissue repair. Cerium (Ce)-doped 45S5 BG (BG/Ce), in particular, offers therapeutic benefits by enhancing osteoblast proliferation and differentiation, as well as providing antimicrobial and antioxidant effects. This study aimed to evaluate the effects of incorporating BG/Ce into alginate-gelatin (ALG-GEL) scaffolds at varying concentrations (5, 10, and 15% w/v), focusing on their physicochemical, biological, antimicrobial, and antioxidant properties to identify the optimal scaffold composition. Materials and Methods: In this experimental study, BG and Ce-doped BG were synthesized and characterized. ALG-GEL, ALG-GEL-BG, and ALG-GEL-BG/Ce scaffolds were fabricated at varying concentrations and subjected to physicochemical analyses. Cytotoxicity was evaluated using an MTT assay at 48 and 72 hours, while cell morphology was examined by scanning electron microscopy (SEM). The antimicrobial activity of selected scaffolds (ALG-GEL, ALG-GEL-BG 10%, and ALGGEL- BG/Ce 10%) was assessed via optical density measurements at 620 nm (OD-620), field emission SEM (FE-SEM), and disk diffusion tests. Antioxidant capacity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: compared to its Ce-free counterpart. Furthermore, the ALG-GEL-BG/Ce 10% scaffold exhibited notable antioxidant activity. Conclusion: The ALG-GEL-BG/Ce scaffold demonstrates enhanced physicochemical, antibacterial, and antioxidant properties, highlighting its potential as a novel biomaterial for bone regeneration applications.
Source: PubMed
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7130845698
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