Work

Generation of a homozygous TET2 knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential (CHIP)

Year: 2026

Type: article

Abstract: Atherosclerotic cardiovascular diseases (CVD) have been linked to mutations related to clonal hematopoiesis of indeterminate potential (CHIP), with Tet methylcytosine dioxygenase 2 (TET2) being the second most often altered gene. (Jaiswal et al. 2017; Abplanalp et al. 2021) We produced a genome-edited human induced pluripotent stem cell (hiPSC) line with a homozygous knock-out (KO) of TET2, simulating loss-of-function mutations, and a control line. We evaluated the pluripotency status of these lines and their capacity to differentiate into mesoderm, ectodem and endoderm. Analysis of the cellular pathomechanisms of TET2-related, CHIP-associated CVDs is made possible by the generation of these cell lines.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2026.103942

Authors: Christos Triantafyllou, Michael Peitz, Bernd K. Fleischmann, Sarah Rieck

Institution: University of Bonn

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,360