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Utilization of BIO-PCR to detect viable Diaporthe destruens from sweet potato tuber in seed tuber production

Year: 2026

Type: article

Abstract: Real-time PCR is highly specific and sensitive for Diaporthe destruens, which causes foot rot of sweet potato. However, PCR detects pathogens whether they are viable or not. We utilized BIO-PCR to detect viable D. destruens from sweet potato tubers. BIO-PCR increased detection sensitivity of the viable pathogen but not the nonviable. Thus, BIO-PCR could be useful to avoid false positive results, which arise from detection of nonviable pathogen.

Source: 日本植物病理學會報

DOI: 10.3186/jjphytopath.92.14

Authors: Y. TSUKIORI, Y. INOUE

Institutions: Ministry of Agriculture, Forestry and Fisheries, National Agriculture and Food Research Organization

Language: English

Topic: Plant Pathogens and Fungal Diseases

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

OpenAlex ID: W7131268613

FWCI:

Cites: 15

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