Work

Negative selection assisted isolation of a highly selective DNA aptamer for the detection of 17α-ethinylestradiol

Year: 2026

Type: article

Abstract: of 200 nM based on thioflavin T (ThT) fluorescence spectroscopy and 138 nM based on isothermal titration calorimetry. Using this ThT fluorescence assay, a limit of detection of 40 nM was determined, and its binding to E2 was much weaker. This aptamer provides a promising molecular recognition element for the development of biosensors and assays capable of detecting trace levels of EE2.

Source: The Analyst

DOI: 10.1039/d5an01374g

Authors: Nathania Lui, Qiushi Liu, Juewen Liu

Institution: University of Waterloo

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: Government of Canada, Canada Research Chairs, Natural Sciences and Engineering Research Council of Canada