Work

Constructing Haloarchaeal MVA Pathway in Serratia marcescens for Synthesis of Ocimene

Year: 2026

Type: article

Abstract: ABSTRACT Ocimene, a valuable monoterpene with applications in agriculture, pharmaceuticals, and fragrances, is challenging to produce at high yields using traditional microbial platforms such as Escherichia coli and Saccharomyces cerevisiae due to monoterpene toxicity. In this study, Serratia marcescens , known for its tolerance to organic solvents and terpenes, was engineered to synthesize ocimene through the haloarchaeal mevalonate (MVA) pathway. We optimized key enzymes, promoters, and pathway construction to enhance production. The engineered strain successfully produced ocimene, with a yield of 436 mg·L −1 in a 50 mL shake‐flask and 2.4 g·L −1 in a 5 L fermenter, demonstrating the potential of S. marcescens as an efficient platform for industrial‐scale ocimene production.

Source: Biotechnology and Applied Biochemistry

DOI: 10.1002/bab.70151

Authors: Songsong Yao, Di Liu, Long Wang, TaiPing Fan, Huaxiang Deng, Yujie Cai

Institutions: Jiangnan University, University of Cambridge, Fuyao University of Science and Technology, Jilin University

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7131808201

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