Human Umbilical Cord Perivascular Cells (HUCPVCs): a mesenchymal cell source for dermal wound healing
Year: 2008
Type: dissertation
Abstract: Mesenchymal stern cells, derived from bone marrow (BM-MSC), have been used with clinical success to enhance the healing of acute and chronic dermal wounds. However, BM-MSC procurement is limited due to the necessity for volunteers of appropriate age. Human Umbilical Cord Perivascular Cells (HUCPVCs) represent an alternative, non-controversial source of postnatal progenitor cells. The goal of the present work was to evaluate the effectiveness of PKH-67 labeled 1PUCPVCs to repair full thickness excisional skin wounds in an immunocompromised mouse model by their direct delivery using a fibrin sealant. Macroscopically and microscopically, HUCPVC treated defects at both 3 and 7 days demonstrated enhanced wound closure, a thicker and more organized dermal granulation tissue and complete re-epithelialization when compared to fibrin only control defects. Moreover, HUCPVC treated defects significantly increased the wound tensile strength over untreated controls. K167 labeling of cell derived from wound digests demonstrated unequivocably that HUCPVCs were mitotically active in-vivo at 5 days. A diffusion chamber model demonstrated HUCPVCs were capable of synthesizing both type I and III collagens in-vivo at 2 weeks. These data provide the first evidence of the efficacy of HUCPVCs to enhance early dermal wound healing suggesting their potential use in both acute and chronic dermal tissue engineering strategies.
Source: TSpace (University of Toronto)
Author: Zebardast, Nazlee
Topic: Wound Healing and Treatments
Subfield: Rehabilitation
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7133016185
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