Work

Systematic Review: Long-Read Sequencing in Algal Studies

Year: 2026

Type: review

Abstract: Long-read sequencing (LRS) has transformed life science research by introducing third-generation sequencing (TGS) platforms applicable across various research fields, including environmental sciences. In the past decade, LRS platforms have been utilized to extensively study algal systems by improving genomic approaches such as metabarcoding, chromosome-level genome and pangenome assemblies, as well as providing new insights into algae-associated microbiomes and host-symbiont interactions. This review aims to discuss recent advancements in LRS in algal research. To achieve this aim, a systematic review was conducted according to the PRISMA 2020 guidelines and across three electronic databases (Web of Science, Scopus, and Google Scholar), with additional citation searching for relevant studies in four key algal research areas: metabarcoding, genomics, pangenomics, and host-symbionts interactions. Following the inclusion and exclusion criteria, only 51 studies were selected for this review. Throughout the review, we summarize the challenges of short-read sequencing (SRS) and discuss how LRS platforms address these challenges in algal studies. Furthermore, we discuss the future of LRS and explore how artificial intelligence (AI) can advance research on algal biology and ecology.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms27052415

Authors: Kakima Kastuganova, Alyamdar Askerov, Attila Szabó, Natasha S. Barteneva

Institutions: Nazarbayev University, Swedish University of Agricultural Sciences

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,499

Funder: Nazarbayev University