Heterologous Production of 2,2′-Dihydroxy Derivatives of Astaxanthin and Adonirubin in Escherichia coli and Evaluation of Their Antioxidant Activity
Year: 2026
Type: article
Abstract: Astaxanthin is a prominent carotenoid with strong antioxidant activity due to its 13 conjugated double bonds and its 3,3′-hydroxy groups adjacent to its 4,4′-carbonyl groups. This red pigment is utilized as a food additive and nutritional supplement, and it also has applications in cosmetics. But the extremely low water solubility of astaxanthin limits its broader commercial application. In order to decrease the hydrophobic property of astaxanthin, we produced 2,2′-dihydroxy derivatives of astaxanthin and its intermediate adonirubin, (2R,3S,2′R,3′S)-2,2′-dihydroxyastaxanthin (1) and (2R,3S,2′R)-2,2′-dihydoxyadonirubin (2), in the cells of Escherichia coli as dominant carotenoids. This result was achieved by using the crtG gene that codes for zeaxanthin/canthaxanthin/astaxanthin 2,2′-hydroxylase, derived from Brevundimonas sp. strain SD212, in addition to astaxanthin biosynthesis genes that carry the Haematococcus pluvialis IDI, Pantoea ananatis crtE, crtB, crtI, crtY, crtZ, and Paracoccus sp. N81106 crtW genes. The singlet oxygen-quenching activities of 1 and 2 (IC50 4.3 μM and 8.3 μM, respectively) were examined and found to be comparable to that of astaxanthin (IC50 1.7 μM).
Source: Antioxidants
Institutions: Japan Women's University, Ishikawa Prefectural University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,499
OpenAlex ID: W7133854028