Polar microcystins or arginine methyl ester can serve as sensitive reference materials for system suitability tests in untargeted metabolomics using reversed-phase LC-MS: A case study
Year: 2026
Type: article
Abstract: Introduction LC-MS system suitability test (SST) is crucial for reliable data acquisition especially in untargeted metabolomics. Objectives Identification of best reference materials (RMs) to improve best quality assurance (QA) and quality control (QC) practices. Methods Investigations were performed using a C 18 reversed-phase (RP) column LC-MS approach. Results Targeted cyanotoxin analysis revealed a performance loss of the used C 18 RP column although the SST confirmed a fit for purpose instrument which prompted to test several additional RMs. Conclusion QA procedures for LC-MS can be improved by incorporating polar microcystins or arginine methyl ester as RMs for SST.
Source: Metabolomics
Authors: Tommy Melzer, Georg Pohnert, Nico Ueberschaar
Institution: Friedrich Schiller University Jena
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,890
Awards: G1406299232, G281949974, EXC 2051: Balance of the Microverse, SFB 1127: Chemische Mediatoren in komplexen Biosystemen (ChemBioSys), ThWIC: Erforschung einer automatisierten, modularen und skalierbaren Probenvorbereitungsplattform für variable Analyseverfahren am Beispiel der Identifizierung von umwelt- und chemisch-ökologisch relevanten Substanzen und Metallen in Gewässern (A2: VANAPLA) - B, G80795443
OpenAlex ID: W7134140341