miR-210 locus deletion disrupts cellular homeostasis an integrated genetic study
Year: 2026
Type: dataset
Abstract: MiR-210 is widely recognized as the quintessential hypoxia-responsive miRNA and is thought to fine-tune various facets of cellular homeostasis. We hereby present an integrative appraisal of the phenotypic and molecular repercussions of disrupting the corresponding locus in human and mouse cells using multiple genetic strategies. In brief, MIR210 deletion led to decreased cellular fitness and suboptimal responses to several stress types. Transcriptomic comparisons via different profiling platforms, performed independently by members of this collaboration, revealed consistent deregulation of neighboring genes, in locus-disrupted cells. Interestingly, the anticipated enrichment of miR-210 targets failed to materialize in unbiased analyses. Our results point to the biological significance of unrecognized regulatory elements that overlap miRNA genes and should serve as a note of caution for studies based on the genetic disruption of such loci. Keywords: Gene editing technologies; Genomic neighborhood; Hypoxia; Targets; miR-210.
Authors: Preda, Mihai Bogdan, Nastase-Rusu, Evelyn Gabriela, Neculachi, Carmen Alexandra, Zhong, Xiaoling, Voellenkle, Christine, Mazure, Nathalie M, Balacescu, Ovidiu, Ivan, Cristina, Zheng, Xiao-Wei, Gherghiceanu, Mihaela, Lebrigand, Kevin, Simionescu, Maya, Martelli, Fabio, Mari, Bernard, Catrina, Sergiu-Bogdan, Burlacu, Alexandrina, Ivan, Mircea
Institutions: Indiana University Indianapolis, Indiana University School of Medicine, IRCCS Policlinico San Donato, Université Côte d'Azur, Centre Méditerranéen de Médecine Moléculaire, Institute of Oncology Prof. Dr. Ion Chiricuta, The University of Texas MD Anderson Cancer Center, Caris Life Sciences (United States), Karolinska Institutet, Carol Davila University of Medicine and Pharmacy, Institutul National Victor Babes, Centre National de la Recherche Scientifique, Inserm, Institut de Pharmacologie Moléculaire et Cellulaire
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7134855947