Work

Mechanistic Studies on DNA Damage via Independent Generation of Purine Reactive Intermediates

Year: 2026

Type: article

Abstract: DNA oxidation is a collection of complex processes resulting from myriad reactive intermediates. That DNA is a heterogeneous polymer and is noncovalently bound to octameric protein complexes in the nuclei of mammalian cells, presents additional challenges when carrying out mechanistic studies. Experiments on nucleosides and oligonucleotides that employ photochemical precursors to reactive intermediates have provided valuable mechanistic insight into these biologically important chemical reactions. Experiments with these molecules have facilitated resolving mechanistic questions and uncovered new chemical pathways. In this review, studies in which independently generated purine nucleobase radicals are employed are summarized.

Source: Journal of Physical Organic Chemistry

DOI: 10.1002/poc.70075

Authors: Liwei Zheng, Marc M. Greenberg

Institutions: Stanford Medicine, Stanford University, Johns Hopkins University

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Diverse Nucleic Acids Chemistry: FundamentalStudies and Applications., G992093831