Work

Discovery and engineering of bacterial P450s for C-14 hydroxylation in ent-kaurane diterpenoids

Year: 2026

Type: article

Abstract: ent-Kaurane diterpenoids (ent-KTs) represent a structurally diverse class of natural products renowned for their antitumor and anti-inflammatory bioactivities. The C-14 hydroxyl modification is crucial for enhancing their potency; however, introducing this functional group remains a considerable challenge. Here, we present a computational heme-guided site-specific (CHS) strategy to identify three bacterial P450s (CYP260A1, CYP105N1, and CYP154C5) for C-14 hydroxylation. Further computationally guided enzyme engineering and redox partner screening identify the CYP260A1 L162V variant paired with CamA/CamB, achieving a 52-fold increase in production titer and a yield of 84.2 mg/L of (14R,16R)-ent-kauran-14,16-diol (2) in Escherichia coli. Substrate scope test reveals functional groups affecting reactivity. Structure-activity relationship studies demonstrate the synergistic effect between the C-14 hydroxyl and C15–C16 Michael acceptor, resulting in a potent derivative (27) with strong cytotoxicity (IC50HCT116 = 1.4 μM). This study demonstrates a framework combining CHS-guided P450 discovery and computational enzyme engineering to advance ent-KT modifications. C-14 hydroxyl group is critical for the function of ent-kaurane diterpenoids (ent-KTs). Here, the authors report discovery and engineering of bacterial P450s for C-14 hydroxylation in ent-KTs.

Source: Nature Communications

DOI: 10.1038/s41467-026-70157-0