Work

Whole-genome sequencing of Escherichia coli DH5α using MinION nanopore technology

Year: 2026

Type: article

Abstract: ABSTRACT We present the whole-genome sequence of Escherichia coli DH5α, a laboratory strain integral to molecular biology applications. We assembled a high-quality genome using Qiagen’s DNeasy Blood and Tissue Kit and the Oxford Nanopore MinION platform (~4.58 Mb, 50.8% guanine-cytosine [GC] content), offering insights into genetic structure and functional characteristics.

Source: Microbiology Resource Announcements

DOI: 10.1128/mra.01076-25

Authors: Megha S. Kumar, P.V. Suneesh, Manoj Bhat Krishna, K.P. Soman, T.G. Satheesh Babu

Institution: Amrita Vishwa Vidyapeetham

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: Amrita Vishwa Vidyapeetham University

OpenAlex ID: W7135047686

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Cites: 10

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