Work

Overcoming isolation barriers: in vitro cultivation and molecular identification of Cephaleuros virescens associated with African mahogany

Year: 2026

Type: article

Abstract: Algae of the genus Cephaleuros are specialized plant pathogens prevalent in tropical ecosystems; however, in vitro studies remain constrained by the historical difficulty of establishing axenic cultures. This study aimed to isolate, identify, and establish a functional cultivation protocol for Cephaleuros associated with algal leaf spot on African mahogany ( Khaya ivorensis ) in the Brazilian Cerrado. Isolation was successfully achieved through a phased liquid-to-solid transition: symptomatic leaf fragments were initially cultivated in liquid Trebouxia medium under constant agitation (120 rpm) for 3 days, followed by the subculturing of algal mycelium onto Trebouxia agarized medium to obtain pure colonies of isolate H-27-03. Direct isolation on solid media failed across all tested substrates (PDA, PSA, BBM, and HLE), confirming the requirement for a liquid phase to facilitate sporangia release. Surface disinfestation of host tissue proved critical, significantly enhancing algal development (yielding a growth score of Note 2) compared to non-disinfested fragments (Note 1). Phylogenetic analysis of the 18S rRNA gene sequence (GenBank MH042530) confirmed the species as Cephaleuros virescens with >98% identity. This study represents the first report of C. virescens causing algal leaf spot on K. ivorensis in the Brazilian Cerrado. By overcoming isolation barriers through standardized nutritional and physical parameters, this work provides a fundamental framework for future research on the epidemiology, physiology, and management of this emerging forest pathogen.

Source: Frontiers in Plant Science

DOI: 10.3389/fpls.2026.1794889

Authors: Fabíola Teodoro Pereira, Natália Cassia de Faria Ferreira, Ângelo Márcio da Silva Fuzzo, Thiago Alves Santos de Oliveira, Elizabeth Amélia Alves Duarte, Daniel Diego Costa Carvalho

Institutions: Universidade Estadual de Goiás, Universidade Federal do Oeste da Bahia

Language: English

Topic: Plant Pathogens and Fungal Diseases

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,801