Biotic-response networks are an important organizer of the transcriptome in wild Arabidopsis thaliana populations
Year: 2026
Type: preprint
Abstract: Extensive laboratory experimentation has revealed conserved molecular pathways controlling growth and stress responses in plants, yet how these programs operate in natural settings remains poorly understood. We investigated transcriptome organization in wild populations of Arabidopsis thaliana by sampling plants from 60 natural sites in Europe and North America across two seasons. Transcriptomes varied extensively among individuals and showed largely continuous rather than discrete structure across geography and season. Although disease and microbial colonization were common in the wild, wild transcriptomes did not simply recapitulate canonical laboratory stress signatures. Measured microbial infection, environmental, and phenotypic variables explained only a modest fraction of total expression variation, but infection-associated signals accounted for the largest share of the explainable component. Consistent with this, biotic-response networks defined in controlled laboratory experiments were well conserved in wild transcriptomes, whereas control and abiotic-response networks were substantially reorganized. Together, these results suggest that while core transcriptional modules remain recognizable across environments, regulatory relationships among modules differ markedly between laboratory and natural contexts. Graphical Abstract
Institutions: Johann Heinrich von Thünen-Institut, Max Planck Institute for Biology, New York University, Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Laboratoire des Interactions Plantes Microbes Environnement, Université de Toulouse, Seoul National University, University of Tübingen
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Life on land
Open Access status: green
OpenAlex ID: W7135181555