Work

Protocol for targeted peripheral adeno-associated viral vector transduction of the intradental neurons in mice

Year: 2026

Type: article

Abstract: Here, we present the methodology for surgical preparation of the mouse molar teeth and subsequent adeno-associated virus (AAV) transduction of intradental neurons within the tooth (AAV-i.d.). We describe steps for mouse positioning, microscope setup, drilling to expose pulp horns, and applying the AAV-silk mixture. We then detail procedures for composite placement to cover the preparation and trigeminal ganglion (TG) screening to visualize the surface and assess and verify AAV labeling. AAV-i.d. labels intradental neurons and traces their associated neuronal projections. For complete details on the use and execution of this protocol, please refer to Ronan et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104424

Authors: Ling-Yu Liu, Amy Chang, Isaac T. Berthaume, Joshua J. Emrick

Institution: University of Michigan

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550