Work

Effects of Cannabidiol on TAFAZZIN ‐Deficient B‐Lymphoblastoid Cells

Year: 2026

Type: article

Abstract: ABSTRACT Barth Syndrome (BTHS) is a debilitating X‐linked genetic disorder caused by mutations in the gene encoding TAFAZZIN, an enzyme responsible for the remodeling of cardiolipin. While cyclic neutropenia is a well‐recognized immunological feature of this disease, emerging evidence suggests that lymphopenia may also occur. The objective of this study was to examine the effects of cannabidiol (CBD) on growth, cardiolipin content, and mitochondrial abnormalities in BTHS patient‐derived B‐lymphoblastoid cells. CBD (1 μM) restored the growth of BTHS B‐lymphoblastoids to healthy control levels, but did not alter cell cycle distribution or sub‐G1 cell populations, which surprisingly also did not differ from healthy control B‐lymphoblastoids. CBD treatment also fully restored the total cellular cardiolipin concentration and reversed the elevation in monolysocardiolipin/cardiolipin ratio in BTHS B‐lymphoblastoids to healthy cell levels, but did not restore the cardiolipin fatty acyl composition. Assessment of mitochondrial markers suggested that increased cardiolipin did not result from increased mitochondrial content. This improvement in cardiolipin concentration was associated with a significant increase in the maximal coupled state III respiration of BTHS B‐lymphoblastoids, with all five tested BTHS donors exhibiting increased mitochondrial membrane potential following CBD treatment. CBD fully reversed the deficit in succinate dehydrogenase subunit A in BTHS cells, and partially reversed deficits in cytochrome c oxidase subunits I and IV, and partially restored supercomplex I/III 2 levels, but did not rescue I/III 2 /IV levels. This work suggested a potential role for CBD as a therapeutic in BTHS B‐lymphopenia that merits further investigation.

Source: The FASEB Journal

DOI: 10.1096/fj.202503384r