Work

Rapid Quantitative Imaging of Heterogeneous Tissue Hemoglobin Dynamics Using Spatial Frequency Domain Imaging

Year: 2026

Type: article

Abstract: Introduction: Arterial insufficiency is a key factor in chronic wounds, diabetes, and peripheral arterial disease, all of which impair vascular function. Accurate monitoring of tissue-level oxygenation and hemodynamics is critical for assessing outcomes in cases of vascular compromise. However, many existing tools only measure small, localized regions of tissue. This study evaluates spatial variation in oxygenation during a vascular occlusion test (VOT) using wide-field spatial frequency domain imaging (SFDI). METHODS: ) and total hemoglobin in the papillary dermis (HbT1) and deeper tissue (HbT2) during a VOT in 13 subjects. Measurements were taken immediately before induction of the occlusion, after 4 min of occlusion, and immediately after occlusion release. Two regions of interest (ROIs) were analyzed: (1) areas with larger subsurface vessels (macrovasculature) and (2) areas dominated by capillary networks (microvasculature). RESULTS: changes during occlusion, indicating higher oxygen extraction. HbT1 concentrations did not differ significantly between ROIs at any time point. CONCLUSION: Spatial variation is critical when comparing tissue hemodynamics across time and subjects. Noncontact wide-field imaging enables assessment of heterogeneous tissue regions that are difficult to evaluate with probe-based methods. .

Source: Journal of Vascular Research

DOI: 10.1159/000551348

Authors: Gordon T. Kennedy, Rebecca Rowland, Amaan Mazhar, David J. Cuccia, Anthony J. Durkin

Institutions: University of California, Irvine, Beckman Laser Institute and Medical Clinic, Modulim (United States)

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid