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Sustainable Encapsulation of Biocontrol Agents: Cross-Linker Influence on Carboxymethylcellulose-Based Microbeads

Year: 2026

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide The use of biopolymers for encapsulating active ingredients is a well-established approach, with ionotropic gelation representing a viable technique. This method allows the use of various cross-linking agents, though the physicochemical properties of the resulting materials can vary depending on the cross-linker selected. This study aimed to evaluate calcium (Ca 2+ ) and aluminum (Al 3+ ) ions as cross-linkers for the formation of carboxymethylcellulose (CMC) microbeads that can carry biological agents. Following comparative analyses, the most effective cross-linker was used for encapsulating the entomopathogenic fungus Beauveria bassiana strain IBCB66. Encapsulation of B. bassiana within a biopolymer bead matrix was found to be a promising strategy to preserve its biological control properties. Beads cross-linked with Al 3+ (CMC_Al 3+ ) demonstrated superior thermal stability ( T _max of 165.76 and 386.71 °C) and swelling capacity (≈800%) compared to those cross-linked with Ca 2+ ( T _max of 211.78, 223.22, 309.29, and 368.95 °C, and swelling capacity of ≈200%). CMC_Al 3+ beads also exhibited a uniform average size (1.92 ± 0.11 mm), in contrast to the heterogeneous conglomerates observed in CMC_Ca 2+ beads. Blastospores of B. bassiana were efficiently encapsulated in CMC_Al 3+ beads via a simple and rapid method, with 85% germination observed on the bead surface after five months of storage at −18 °C. These findings indicate that aluminum is a promising cross-linking agent for CMC-based encapsulation matrices in biological control applications.

Source: ACS Omega

DOI: 10.1021/acsomega.5c06970