Work

Electrochemical glucose sensing using an electrochemically reduced graphene oxide/poly-L-lysine composite electrode modified with glucose oxidase

Year: 2026

Type: article

Abstract: Facilitating efficient electrical communication between glucose oxidase (GOx) and electrode surfaces is important for the development of low-potential reagent-free glucose biosensors, yet unequivocal direct electron transfer (DET) for native GOx remains controversial because the FAD redox center is deeply buried within the protein structure. In this work, a one-step electrochemical co-deposition strategy was used to fabricate an electrochemically reduced graphene oxide/L-lysine (ERGO–Lys) nanocomposite on glassy carbon. The resulting interface exhibited a pair of well-defined redox peaks centered at −0.460 V (vs Ag/AgCl) and glucose-responsive behavior under rigorously deoxygenated conditions. Given the continuing debate surrounding native GOx DET at carbon nanomaterials, these results are interpreted conservatively as DET-like (pseudo-DET) bioelectrochemistry rather than definitive proof of true DET. Under anaerobic conditions, the biosensor showed a linear range of 5 μM to 9.0 mM, a sensitivity of 35.8 μA·mM⁻¹·cm⁻², and a limit of detection of 1.8 μM. The sensor also retained 90% of its initial response after two weeks and showed low interference at −0.40 V.

Source: International Journal of Electrochemical Science

DOI: 10.1016/j.ijoes.2026.101357

Authors: Xiaofei Zhang, Jingfan Xin, Ruifa Jin

Institutions: Chifeng University, Hechi University

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,670

Funder: Natural Science Foundation of Inner Mongolia