Additional file 1: Figure S1. of Hybrid de novo genome assembly and centromere characterization of the gray mouse lemur (Microcebus murinus)
Year: 2017
Type: article
Abstract: Detailed flowchart of methods used herein for the de novo assembly of the gray mouse lemur (Microcebus murinus). The initial assembly was generated using Illumina data and AllPaths-LG, followed by refined scaffolding using Atlas-Link and gap filling using Atlas-GapFill. Further gap filling with PacBio data and PBJelly followed generating Mmur 2.0. The Mmur 2.0 assembly was super-scaffolded in an iterative method using BNG optical map data to identify conflicts, break scaffolds and join other scaffolds, and identify Lachesis and Hi-C proximity ligation data to further super-scaffold. The PBJelly method was used a second time to fill gaps in the final super-scaffolds, creating the Mmur 3.0 assembly. Asterisks indicate PBJelly2 and Pilon used the PacBio and Illumina datasets at the top of the diagram, respectively. (PDF 1157 kb)
Source: Figshare
Authors: Larsen, Peter, Harris, R., Liu, Yue, Murali, Shwetha, Campbell, C., Brown, Adam, Sullivan, Beth, Shelton, Jennifer, Brown, Susan, Raveendran, Muthuswamy, Dudchenko, Olga, Machol, Ido, Durand, Neva, Shamim, Muhammad, Aiden, Erez, Muzny, Donna, Gibbs, Richard, Yoder, Anne, Rogers, Jeffrey, Worley, Kim
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7135401353