Work

Single-cell transcriptomics reveals that air-liquid interface culture promotes goblet cell differentiation and inhibits glycolysis in organoid cell monolayers

Year: 2026

Type: article

Abstract: Using single-cell RNA sequencing, this study shows that air-liquid interface (ALI) culture enhances secretory lineage differentiation of intestinal organoid cell monolayers and improves transcriptomic similarity to the native epithelium. ALI reduced hypoxia-associated gene expression and better recapitulates in vivo-like cell-cell interactions, supporting its value for modeling intestinal epithelial heterogeneity in organoids.

Source: American Journal of Physiology-Gastrointestinal and Liver Physiology

DOI: 10.1152/ajpgi.00251.2025

Authors: Tania Malonga, Emeline Lhuillier, Christelle Marrauld, Deborah Fourmy, Elodie Riant, Cedric Cabau, Nathalie Vialaneix, Martin Beaumont

Institutions: École Nationale Vétérinaire de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université de Toulouse, Université Toulouse III - Paul Sabatier, Inserm, Genotoul coordination, Génome et Transcriptome - Plateforme Génomique

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Agence Nationale de la Recherche

Award: Gut microbiota-derived metabolites: natural products to promote epithelial barrier maturation at the suckling-to-weaning transition

OpenAlex ID: W7135384499

FWCI:

Cites: 62

Cited by:

Related to: