Work

Time‐ and space‐resolved transcriptional regulation in Arabidopsis thaliana

Year: 2026

Type: article

Abstract: General metabolism and responses to internal or external signals are tightly regulated in plants. We hypothesise that a network of intermediate regulatory proteins including transcription factors, kinases, and E3 ligases connect input signals like light, temperature, circadian clock, and ontogenetic pathways to output pathways. We therefore investigate the transcriptional dynamics of these regulators. RNA sequencing was performed on 3-week-old Arabidopsis thaliana Col-0 rosettes grown under 12 h/12 h light/dark conditions sampled every 2 h over 24 h to match publicly available single-cell data of same age plants. Both were analysed to identify the abundance of intermediate regulators in time and space. Intermediate regulators are of significantly lower abundance compared with other transcripts in the Arabidopsis transcriptome. More than half of expressed kinases, E3 ligases and transcriptions factors vary in either time, space or both in mature leaves. Dynamic expression patterns of regulators allow plants to maintain tightly regulated metabolism while providing sufficient room for specific stress responses. High plasticity of the Arabidopsis transcriptome highlights the importance of considering sampling time-of-day and cellular resolution for experiments.

Source: Plant Biology

DOI: 10.1111/plb.70196

Authors: S. Zenker, K. Schiller, A. Bräutigam

Institution: Bielefeld University

Language: English

Topic: Plant Molecular Biology Research

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,400

Funder: Deutsche Forschungsgemeinschaft