Additional file 1: Figure S1. of Phosphorylation of tau at Y18, but not tau-fyn binding, is required for tau to modulate NMDA receptor-dependent excitotoxicity in primary neuronal culture
Year: 2017
Type: article
Abstract: DNQX and TTX were bioactive. (A–C) Experiments illustrating effects of DNQX (A) and TTX (B, C). (A) A single experiment was performed to confirm that DNQX blocks AMPA-induced neurotoxicity in primary neuronal cultures. Data represent means ± SEM of technical replicates (4–6 wells per condition). WT neurons were treated with vehicle or the competitive AMPAR antagonist DNQX (20 μM) 1 h before and throughout exposure to different concentrations of AMPA. Raw AlamarBlue fluorescence measurements are shown in arbitrary units. (B, C) TTX blocked spontaneous and induced neuronal activity in acute brain slices. Slices of somatosensory cortex were prepared and pyramidal neurons were patch-clamped as described [9]. Whole-cell membrane voltage recordings were made in artificial cerebrospinal fluid (aCSF) in the absence or presence of 3 μM TTX (from the same batch used for cell culture experiments). (B) TTX prevented spontaneous neuronal activity as well as activity induced by 100 pA/200 ms current stimulations (arrowheads) or by a membrane voltage ramp (between asterisks). Scale bar indicates 20 s of recording time. (C) Representative membrane voltage responses elicited by 100 pA/200 ms current stimulations in control aCSF and after addition of 3 μM TTX. (PDF 4818 kb)
Source: Figshare
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7136051727