Work

MOESM3 of Histone peptide microarray screen of chromo and Tudor domains defines new histone lysine methylation interactions

Year: 2017

Type: article

Abstract: Additional file 3: Figure S1. SPIN1 Tudor domain interaction with H3K4me3 and H4K20me3. A) Representative array images of SPIN1 Tudor domain showing peptide binding indicated in red (right panel). The peptide tracer is shown in green (left panel). Positive antibody controls are outlined in white. B) Western blot results of peptide pull-down experiments with SPIN1 tandem Tudor domain. The input is shown in Lane 1 and the corresponding bound fraction is shown in Lanes 2â 8. C) Western blot results of peptide pull-down experiments with whole cell lysates derived from transiently transfected HEK 293T cells (GFP-SPIN1, 2A, 2B, 3 and 4).

Source: Figshare

DOI: 10.6084/m9.figshare.4750123

Authors: Shanle, Erin, Shinsky, Stephen, Bridgers, Joseph, Bae, Narkhyun, Sagum, Cari, Krajewski, Krzysztof, Rothbart, Scott, Bedford, Mark, Strahl, Brian

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7136072812

FWCI:

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