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Additional file 1: Figure S1. of CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Year: 2016

Type: article

Abstract: Optimization of the CEL-Seq protocol. A Number of genes obtained from ten replicates of 100 pg RNA performed with each type of primer: the original primer, the original primer with the inclusion of UMI, and the shortened UMI primer. B Number of transcripts identified for the two primers containing a UMI. C Estimating the efficiency of CEL-Seq using UMIs and ERCC spike-ins. The efficiency is computed as the y-intercept. D Side-by-side comparison of column clean-up, bead clean-up, and two RTs relative to CEL-Seq with a UMI primer. E Side-by-side comparison of different second-strand synthesis enzymes. The MessageAmp II enzyme was the one used originally. (PDF 519 kb)

Source: Figshare

DOI: 10.6084/m9.figshare.4467497

Authors: Hashimshony, Tamar, Senderovich, Naftalie, Avital, Gal, Klochendler, Agnes, de Leeuw, Yaron, Anavy, Leon, Gennert, Dave, Li, Shuqiang, Livak, Kenneth, Rozenblatt-Rosen, Orit, Dor, Yuval, Regev, Aviv, Yanai, Itai

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7136186408

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