Additional file 1: of Generation and characterization of a SIVmac239 clone corrected at four suboptimal nucleotides
Year: 2015
Type: article
Abstract: Figure S1. Possible models for the various proportions of the suboptimal, optimal and mismatched integration within the PBS. Following initial integration, the proportion of suboptimal thymine/adenine (T/A) integrated in the genome is 50% whereas the optimal cytosine/guanine (C/G) is found at only 10% and the uncorrected mixed base of cytosine/adenine (C/A) is found at 40%. There are two possible models that explain the increases proportion of the suboptimal T/A integration; (A) an alternative tRNA priming where a minor tRNA Lys 5 molecule initiates the RT event producing a T/A genome, and/or (B) a reduction in the processivity of RT that prematurely terminates the second strand synthesis prior to the mismatch in the PBS which then requires utilizing the 5â ˛ PBS from the first strand allowing for the incorporation of a suboptimal T/A integrant. To account for the 10% optimal C/G genome, we propose a mechanism whereby an early truncation of first strand synthesis prior to the mismatch will utilize the second strand cytosine for the final template producing an optimal C/G form (panel C).
Source: Figshare
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7137347150